Supplementary information to the manuscript:

Transcript Level Alterations Reflect Gene Dosage Effects Across Multiple Tissues in a Mouse Model of Down Syndrome

Pascal Kahlem^1*, Marc Sultan^1*, Ralf Herwig¹, Matthias Steinfath¹, Daniela Balzereit¹, Barbara Eppens¹, Nidhi G. Saran², Mathew T. Pletcher^2,3, Sarah T. South², Gail Stetten², Hans Lehrach¹, Roger H. Reeves², Marie-Laure Yaspo¹

Affiliations for Authors:
¹ Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195, Berlin, Germany
² Departments of Physiology, Obstetrics and Gynecology, Johns Hopkins Univ. School of Medicine, Baltimore, MD 21205
³ Current address: Scripps Research Institute, La Jolla, CA 92037
^* the first 2 authors should be regarded as equal first authors.

Supplementary Information:

• Suppl. Table 1: Complete raw data for the mmu21 genes (EXCEL file in zipped format)
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• Suppl. Table 2: array data for the Unigene sample
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• Suppl. Table 3: Real time PCR primer sequences
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• Suppl. Figure 1: Comparison of the expression of genes detected by arrays and real time PCR
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• Suppl. Figure 2: Comparison of trisomic/euploid ratios obtained by real-time PCR and arrays - Figure 2.a: only Cerebellum
  - Figure 2.b: only Cortex
  - Figure 2.c: only Heart
  - Figure 2.d: only Kidney
  - Figure 2.e: only Liver
  - Figure 2.f: only Lung
  - Figure 2.g: only Midbrain
  - Figure 2.h: only Skeletal Muscle
  - Figure 2.i: only Testis
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• Suppl. Figure 3: Interactive dendrogram
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• Suppl. Protocols (WORD format): All protocol details of the material and methods
• Array data in the Array Express database at EB: Acc. no E-MEXP-102